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For tips on how to prepare the medium check out: How to Prepare MS Medium Murashige and Skoog Medium, commonly abbreviated as MS Medium, is a plant tissue culture medium that is widely used in plant science research and plant biotechnology. It was first developed by Toshio Murashige and Folke Skoog in 1962 and has since become one of the most popular and versatile plant tissue culture media. MS Medium contains a balanced mixture of essential nutrients and vitamins, that promote the growth and development of a wide range of plant species in vitro. It is commonly used for micropropagation, callus induction, somatic embryogenesis, and other plant tissue culture applications. The composition of MS Medium can be customized by adding or omitting specific components to suit different experimental needs. Also known as Basal Medium, MS, MSO, MS-0 -
How to Use PPM to Prepare Plant Cell Cultures What is Plant Preservative Mixture (PPM) ? Plant Preservative Mixture (PPM), is a broad-spectrum biocide for plant tissue culture. PPM™ is the ultimate solution to the never-ending struggle against microbial airborne, waterborne and endogenous contamination. About Plant Preservative Mixture: PPM™ is a heat stable preservative/biocide which can be used to effectively prevent or reduces microbial contamination in plant tissue culture.- At optimum doses, PPM™, which stands for Plant Preservative Mixture™, is an extremely effective Preservative/Biocide, yet does not impair in vitro seed germination, callus proliferation and callus regeneration.
- PPM™ prevents the germination of both bacteria and fungi spores. It is heat stable and therefore can be autoclaved with the media.
- PPM™ can be, and should be used as a standard ingredient in plant tissue culture media, and is also substantially less expensive than commonly used antibiotics.
- While PPM™ was principally designed to inhibit airborne contamination, waterborne contamination and contamination introduced from human contact, it can also -- in many cases -- be used to reduce endogenous contamination.
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6-Benzylaminopurine (6-BAP or BA) is a first-generation synthetic cytokinin, a plant growth regulator that promotes cell division, differentiation, and lateral bud growth. It is widely used in plant tissue culture for shoot proliferation, in agriculture to improve fruit quality, and to delay senescence in plants. Can be stored at room temperature for a 24 month shelf-life. -
Outsider Labs’ pupping cream contains 2% 6-Benzylaminopurine (also referred to as 6-BAP) and 0.0075% Gibberellic acid (also referred to as GA3). These plant hormones are responsible for growth stimulation, cell division, and cell elongation. The cream induces pupping when applied to the axillary buds, apical meristems, or the areoles of cacti. The cream takes 1 to 10 weeks to produce pups in actively growing plants. Pupping is induced around 90% of the time when applied correctly. Pupping cream is shelf-stable and does not need to be stored in a refrigerator.
